Screening and assessment of molecular mechanistic actions of 5-hydroxy-1-methylpiperidin-2-one against free radicals, lung cancer cell line (A549), and binding properties on bovine serum albumin

Abstract Background Natural products play a key role in treating different ailment including diabetes, asthma, skin diseases, and cancer. It is well known that synthetic drugs elicit significant toxicity when used the clinic. A higher drug affinity towards carrier protein Bovine Serum Albumin (BSA) would enhance bioavailability which turn leads to therapeutic efficacy. The focus of present study was investigate antioxidant anti-cancer potential 5-hyrdoxy1-methylpiperidin-2-one (5-HMP) isolated from leaves Tragia involucrata . Methods material In vitro free radical scavenging assays MTT assay were employed assess activity 5-HMP cytotoxicity on lung cancer cell line, A549, respectively. addition, attempts made binding capacity BSA by spectral studies molecular docking. Results data revealed inhibited radicals with an IC 50 value 49.55 ± 0.75 μg/ml comparable values afforded l -ascorbic acid. exhibited dose-dependent A549 cells 30.00 0.55 μg/ml. further induced cycle arrest at S G2/M phase. fluorescence quenching observed increasing concentration 5-HMP, reacts fixed (1.0 μM). indicated constant K = 2.8 1.4 × 10 4 M −1 corresponding energy (ΔG)−6.06 K.cal/mole. Conclusions This paper concluded possesses properties, cytotoxic effects also it good properties bovine serum albumin.